Dynamic kinetic capillary isoelectric focusing: a powerful tool for studying protein-DNA interactions.
نویسندگان
چکیده
A new method called dynamic kinetic capillary isoelectric focusing (DK-CIEF) is presented for the study of protein-DNA interactions. The method is based on CIEF with laser-induced fluorescence-whole column imaging detection in which protein-DNA complexes are separated with spatial resolution while dissociations of the complexes are dynamically monitored using a CCD camera with temporal resolution. This method allows for the discrimination of different complexes and the measurement of the individual dissociation rate constants.
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ورودعنوان ژورنال:
- Analytical chemistry
دوره 79 3 شماره
صفحات -
تاریخ انتشار 2007